Ozonized biochar filtrate effects on the growth of Pseudomonas putida and cyanobacteria Synechococcus elongatus PCC 7942

Abstract Background Biochar ozonization was previously shown to dramatically increase its cation exchange capacity, thus improving nutrient retention capacity. The potential soil application of ozonized biochar warrants the need for a toxicity study that investigates effects on microorganisms. Results In presented here, we found filtrates collected from pine 400 and rogue did not have any inhibitory environmental bacteria Pseudomonas putida, even at high dissolved organic carbon (DOC) concentrations 300 ppm. However, growth Synechococcus elongatus PCC 7942 inhibited by DOC greater than 75 Further tests showed presence some compounds (terephthalic acid p -toluic acid) in filtrate non-ozonized biochar; these were greatly reduced upon wet-ozonization material. Nutrient detection also dry-ozonization enhanced availability nitrate phosphate filtrate, property may be desirable application. Conclusion Ozonized substances can support bacterium putida growth, since detoxifies aromatic molecules. Graphical